Microscopy, Light & Lenses

Table of Contents

Microscopy, Light & Lenses

Resolution & Color

Microscopes

Photons & Detectors

Daylight & Radiance

Light & Sensitivity

Reciprocity & Sensitivity

Color balance

Focus & depth

Distortion & Aberration

NA & Resolution

Objectives Quality

Non bright modes

Resol. Vs. depth

Processing

Confocal

No shadows

Email: cfermin@tulane.edu

Home Page: www.som.tulane.edu/ferminlab/

Other information:
Prepared for class room teaching at Tulane. You may not modified this lecture in any manner or form and may only use it for the purpose for which it was created. No other activity outside academic functions and class room usage is intended or implied.

This presentation reviews basic concepts of microscopy, light transmission and how optics influence the transmission and property of light through those lenses.

The review is not exhaustive and students and trainees with previous training in microscopy are encouraged to contact the author for additional references on each subject.