Microscopy & Fluorescence

Table of Contents

Microscopy & Fluorescence

Autofluorescence

Dichroid Mirrors & Filters

Beads

Dual spectra

Exciter-barrier

FISH

FTIC

DAPI & FTIC

Email: cfermin@tulane.edu

Home Page: www.som.tulane.edu/ferminlab/

Other information:
Prepared for class room teaching at Tulane. You may not modified this lecture in any manner or form and may only use it for the purpose for which it was created. No other activity outside academic functions and class room usage is intended or implied.

This presentation reviews the most common techniques and approaches used for imaging, recording and archiving fluorescent signals from cell, tissue or organ preparations.

The review is not exhaustive and students and trainees with previous training in computer assisted microscopy, digital deconvolution, and related technologies are encouraged to contact the author for additional references on each subject.