Laboratory Measurement of Thyroid Hormones


  1. Measurement of the TSH/T4 relationship. Hyperthyroidism is almost always caused by a hyperactive thyroid gland which is producing too much thyroid hormone (hypothalamic causes for hyperthyroidism are extremely rare). In the typical case, excess free thyroid hormone feeds back on the hypothalamus and pituitary, resulting in very low TSH levels. Thus low TSH is a very good diagnostic indicator for hyperthyroidism. The opposite happens in thyroid-related hypothyroidism: low free thyroid hormone decreases negative feedback resulting in very high TSH. If TSH is low in the setting of low thyroid hormone, the problem lies in the pituitary or hypothalamus.

    At present, most authorities recommend that the initial workup of thyroid status should be done by measuring TSH or TSH in combination with assay of free T4. In the past, TSH and free T4 assays were less reliable, so a number of other thyroid tests have historical significance (see below).

  2. Effects of changes in thyroid-binding proteins. For years, measurement of total T4 (free + bound) in the blood was an important first step in thyroid evaluation. The problem with this test is that changes in the amount of thyroid binding globulin (TBG) in the blood dramatically affect total blood thyroid hormone (since 99% of thyroid hormone is bound to TBG). However, since free T4 and T3 are what feed back and control thyroid hormone levels, the body would adjust thyroid gland output so that free hormone levels remained normal (and thus thyroid hormone activity in the body was normal) even though the blood content of free + bound (total) hormone was elevated. To correct this problem, a test called "T3 resin uptake" was used to measure the number of available binding sites for thyroid hormone in the blood. With this information, the total T4 value could be mathematically corrected for increased or decreased TBG in the blood. The resulting value, called the "Free Thyroxine Index" (FTI) is proportional to the free thyroid hormone level. Though this value is useful diagnostically, it is less accurate than TSH and free T4 measured directly--which are not affected by changes in TBG--and it has been largely superceded.


Thyroid Metabolism Hypo- and Hyperthyroidism

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Last modified: 1/17/97; Author: J. Harrison