S11C25P20-3: Blue arrows point to intra-nuclear inclusions and green arrows point to cytoplasmic inclusions. To the right, many cells show a distinctive pattern in which globules have collected in the cytoplasm. These cytoplasmic changes are interpreted as representing poorly developed viral inclusions (milker’s nodule).

S11C25P20-4: This is an example of lesion of a herpes simplex viral infection. Cytopathic changes are prominent. Many of the cells have pale, or vacuolated, cytoplasm (ballooning degeneration). Near the top of the field, cells, forming a band, are condensed and show increased cytoplasmic acidophilia. They also show pyknosis of nuclei (dyskeratosis of virus-related type). Inflammatory cells among the keratinocytes show fragmentation of their nuclei. Nuclei of keratinocytes are pale and show marginated chromatin.

S11C25P20-5: Cytopathic changes in this lesion of herpes simplex virus infection are prominent. There is marked cytoplasmic pallor. Intra-cellular edema has progressed on the right side of the field to the formation of a lytic defect (cytolysis). Inflammatory cells, apparently neutrophils, show fragmentation of nuclei. Red arrows point to characteristic intra-nuclear inclusions. At the tip of the red arrow on the right, there is a dense, acidophilic condensation at the nuclear membrane that is interpreted as a nucleolus. Apparently, the nucleolus has been pushed aside by the inclusion.